3D Temperature-Controlled Interchangeable Pattern for Size-Selective Nanoparticle Capture.

Patterned surfaces with distinct regularity and structured arrangements have attracted great interest due to their extensive promising applications. Although colloidal patterning has conventionally been used to create such surfaces, herein, we introduce a novel 3D patterned poly(N-isopropylacrylamide) (PNIPAM) surface, synthesized by using a combination of colloidal templating and surface-initiated photoinduced electron transfer-reversible addition-fragmentation chain transfer (SI-PET-RAFT) polymerization. In order to investigate the temperature-driven 3D morphological variations at a lower critical solution temperature (LCST) of ∼32 °C, multifaceted characterization techniques were employed. Atomic force microscopy confirmed the morphological transformations at 20 and 40 °C, while water contact angle measurements, upon heating, revealed distinct trends, offering insights into the correlation between surface wettability and topography adaptations. Moreover, quartz crystal microbalance with dissipation monitoring and electrochemical measurements were employed to detect the topographical adjustments of the unique hollow capsule structure within the LCST. Tests using different sizes of PSNPs shed light on the size-selective capture-release potential of the patterned PNIPAM, accentuating its biomimetic open-close behavior. Notably, our approach negates the necessity for expensive proteins, harnessing temperature adjustments to facilitate the noninvasive and efficient reversible capture and release of nanostructures. This advancement hopes to pave the way for future innovative cellular analysis platforms.

Synthesis of hyperbranched polymer films via electrodeposition and oxygen-tolerant surface-initiated photoinduced polymerization.

HYPOTHESIS: Hyperbranched polymers, not only possess higher functionality, but are also easier to prepare compared to dendrimers and dendric polymers. Combining electrodeposition and surface-initiated photoinduced electron transfer-reversible addition-fragmentation chain transfer (SI-PET-RAFT) polymerization is hypothesized to be a novel strategy for preparing hyperbranched polymer films on conductive surfaces without degassing. EXPERIMENTS: Polymer brush grafted films with four different architectures (i.e. linear, branched, linear-block-branched, and branched-block-linear) were prepared on gold-coated glass substrates using electrodeposition, followed by SI-PET-RAFT polymerization. The resulting film structure and thickness, surface topology, absorption property, and electrochemical behavior were confirmed by spectroscopy, microscopy, microbalance technique, and impedance measurement. FINDINGS: These hyperbranched polymer brushes were capable of forming a thicker but more uniformly covered films compared to linear polymer brush films, demonstrating that hyperbranched polymer films can be potentially useful for fabricating protective polymer coatings on various conductive surfaces.

On the Interfacial Behavior of Catenated Poly(l-lactide) at the Air-Water Interface.

Interfacial properties of polymeric materials are significantly influenced by their architectural structures and spatial features, while such a study of topologically interesting macromolecules is rarely reported. In this work, we reported, for the first time, the interfacial behavior of catenated poly(l-lactide) (C-PLA) at the air-water interface and compared it with its linear analogue (L-PLA). The isotherms of surface pressure-area per repeating unit showed significant interfacial behavioral differences between the two polymers with different topologies. Isobaric creep experiments and compression-expansion cycles also showed that C-PLA demonstrated higher stability at the air-water interface. Interestingly, when the films at different surface pressures were transferred via the Langmuir-Blodgett method, successive atomic force microscopy imaging displayed distinct nanomorphologies, in which the surface of C-PLA exhibited nanofibrous structures, while that of the L-PLA revealed a smoother topology with less fiber-like structures.

Serum levels of miR‐21 and miR‐155 in T2DM + CHD patients and their relationship with lipid me‐tabolism／ / 心血管康复医学杂志

To explore serum levels of miR‐21 and miR‐155 in patients with T2DM complicated CHD and their relationship with lipid metabolism .Methods : A total of 134 T2DM patients treated in our hospital from 2016 to 2017 , were divided into T2DM + CHD group (n＝60) and pure T2DM group (n＝74).Blood glucose and blood lipid levels and serum miR‐21 and miR‐155 levels were measured and were compared between two groups .Results :There were no significant difference in general data , blood pressure , body mass index (BMI) , glycosylated hemo‐globin A1c (HbA1c) , plasma glucose and total cholesterol (TC) between two groups , P＞0. 05 all.Compared with pure T2DM group , there were significant rise in levels of triglyceride (TG) [ (1. 89 ± 0.92) mmol／L vs.(2. 75 ± 1.61) mmol／L] , LDL‐C [ (2.83 ± 0.79) mmol／L vs.(3. 52 ± 1.24) mmol／L] and serum miR‐21 [ (0. 93 ± 0. 15) vs.(1. 86 ± 0.24 )] , and significant reductions in levels of HDL‐C [ (1.35 ± 0. 34 ) mmol／L vs.(0. 94 ± 0.31 ) mmol／L] and serum miR‐155 [ (0. 95 ± 0.19) vs.(0. 27 ± 0. 10)] in T2DM + CHD group , P＝0.001 all.Multiva‐riate Logistic regression analysis indicated that TG , LDL‐C and miR‐21 were independent risk factors for T2DM +CHD (OR＝2. 800～4. 986 , P＜0.05 all) , while HDL‐C and miR‐155 were its independent protective factors (OR＝0.314 , 0.327 , P＜ 0.05 both).Pearson correlation analysis indicated that serum miR‐21 level was significant positively correlated with TG and LDL‐C levels ( r＝0. 415 , 0.506 , P＜0.05 or ＜0. 01) , and serum miR‐155 level was significant inversely correlated with TG and LDL‐C levels ( r＝ －0. 397 ,－0. 526 , P＜0.05 or ＜0. 01 ).Con‐clusion : Serum miR‐21 level was significant positively correlated with TG and LDL‐C levels , but serum miR‐155 level was significant inversely correlated with TG and LDL‐C levels ,

Influence of ticagrelor on myocardial injury in patients with acute myocardial infarction after emergen-cy PCI / 心血管康复医学杂志

Objective :To explore influence of ticagrelor on myocardial injury ,serum levels of N terminal pro brain natriuretic peptide (NT-proBNP) ,tumor necrosis factor (TNF)-α and endothelin (ET) in patients with acute myo-cardial infarction (AMI) after emergency percutaneous coronary intervention (PCI).Methods : A total of 94 AMI patients undergoing emergency PCI were selected ,and were divided into clopidogrel group (n＝ 45 ) and ticagrelor group (n＝ 49 ) ,two groups received postoperative dual-antiplatelet therapy of aspirin combined clopidogrel or ti-cagrelor respectively .After one-month treatment ,index of microcirculatory resistance (IMR ) ,coronary flow re-serve (CFR ) , serum levels of NT-proBNP , TNF-α , ET , and incidence of major adverse cardiovascular events (MACE) within one year were compared between two groups .Results : Compared with clopidogrel group after 12- month treatment ,there were significant reductions in IMR [21-24 ± 4-07 ) vs.(15-33 ± 4-82)] ,serum levels of NT-proBNP [(123-17 ± 16-25) ng／L vs.(63-46 ± 12-13) ng／L] ,TNF-α [(4-04 ± 0-84) mg／L vs.(3-07 ± 0-52) mg／L] ,ET-1 [ (48-71 ± 6-53) ng／L vs.(38-04 ± 5-89) ng／L] ,and significant rise in CFR [ (1-73 ± 0-34) vs. (2-36 ± 0-42)] in ticagrelor group , P＝ 0-001 all .Incidence rate of MACE in ticagrelor group was significantly lower than that of clopidogrel group (6-12% vs .24-44%, P＝ 0-018 ).Conclusion : Compared with clopidogrel group ,ticagrelor group possesses significant therapeutic effect in AMI patients after emergency PCI .And it′s safe .

Star-like copolymer stabilized noble-metal nanoparticle powders.

The amphiphilic star-like copolymer polyethylenimine-block-poly(ε-caprolactone) (PEI-b-PCL) was utilized to transfer the pre-synthesized citrate-capped noble metal nanoparticles (NMNPs) from an aqueous layer to an organic layer without any additional reagents. Dynamic light scattering (DLS) and transmission electron microscopy (TEM) were utilized to study the assembly of the polymers coated on the surface of the citrate-capped NMNPs. After removing the organic solvent, the polymer-coated NMNPs in powder form (PCP-NMNPs) were obtained. The excellent solubility of the PEI-b-PCL allows the PCP-NMNPs to be easily dispersed in most of the organic solvents without any significant aggregation. Moreover, the good thermal stability and long-term stability make PCP-NMNPs an excellent NMNP-containing hybrid system for different specific applications, such as surface coating, catalysis and thermoplastic processing of nanocomposite materials.